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amidinophenyl 6 indolecarbamidine dihydrochloride jo  (Beyotime)


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    Beyotime amidinophenyl 6 indolecarbamidine dihydrochloride jo
    Amidinophenyl 6 Indolecarbamidine Dihydrochloride Jo, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33033 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+dihydrochloride/DAPI/pm42880593-220-1-19
    Average 99 stars, based on 33033 article reviews
    amidinophenyl 6 indolecarbamidine dihydrochloride jo - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: 68 Ga-FAPI-04 PET/CT for the early diagnosis of multi-organ fibrosis and functional impairments caused by platinum-based chemotherapy.
    Article Snippet: 1 Department of Thoracic Surgery, Changzheng Hospital, Naval Medical University, No. 415, Fengyang Road, Huangpu District, Shanghai, China 2 Department of Nuclear Medicine, Changhai Hospital, Naval Medical University, Shanghai, China 3 Department of Cardiothoracic Surgery, People’s Liberation Army Navy Medical Center, Naval Medical University, Shanghai, China 4 Department of Orthopaedic Oncology, Changzheng Hospital, Naval Medical University, Shanghai, China Abstract Purpose Platinum-based chemotherapeutic agents are non-specific anticancer drugs known to induce multi-organ fibrosis and functional impairments.. This study aimed to evaluate multi-organ fibrosis and functional impairments following platinum-based chemotherapy using 68Ga-FAPI-04 PET/CT imaging.. Methods 68Ga-FAPI-04 PET/CT imaging was performed to assess fibroblast activation in both preclinical and clinical settings.

    CCK-8 Assay:

    Article Title: A Multifunctional Nanoplatform for Treating Hepatic Ischemia-Reperfusion Injury by Modulating the Nrf2 and NF-κB Pathways.
    Article Snippet: .. DAPI dihydrochloride, Total Antioxidant Capacity (TAOC) Assay Kit, Cell Counting Kit-8 (CCK-8), and Colorimetric TUNEL Apoptosis Assay Kit were obtained from Beyotime Biotechnology Company (Shanghai, China). ..

    Article Title: Chronic exposure to polystyrene micro/nanoplastics triggers testicular dysfunction through PI3K/AKT/mTOR signaling-mediated spermatocyte senescence in mice.
    Article Snippet: While the potential microplastic toxicity has attracted considerable research attention, studies on its chronic reproductive effects in male mammals remain limited.. Here, we investigated the chronic reproductive toxicity of polystyrene microplastics (PS-MPs, 5 μm) and polystyrene nanoplastics (PS-NPs, 20 nm) both in vitro, using GC2 cells, and in vivo, employing male C57 mice.. Histopathological analysis revealed dilated seminiferous tubules, disorganized spermatocytes, and reduced spermatocyte counts in treated groups.

    Article Title: A Multifunctional Nanoplatform for Treating Hepatic Ischemia–Reperfusion Injury by Modulating the Nrf2 and NF-κB Pathways
    Article Snippet: .. DAPI dihydrochloride, Total Antioxidant Capacity (T-AOC) Assay Kit, Cell Counting Kit-8 (CCK-8), and Colorimetric TUNEL Apoptosis Assay Kit were obtained from Beyotime Biotechnology Company (Shanghai, China). ..

    TUNEL Assay:

    Article Title: A Multifunctional Nanoplatform for Treating Hepatic Ischemia-Reperfusion Injury by Modulating the Nrf2 and NF-κB Pathways.
    Article Snippet: .. DAPI dihydrochloride, Total Antioxidant Capacity (TAOC) Assay Kit, Cell Counting Kit-8 (CCK-8), and Colorimetric TUNEL Apoptosis Assay Kit were obtained from Beyotime Biotechnology Company (Shanghai, China). ..

    Article Title: Chronic exposure to polystyrene micro/nanoplastics triggers testicular dysfunction through PI3K/AKT/mTOR signaling-mediated spermatocyte senescence in mice.
    Article Snippet: While the potential microplastic toxicity has attracted considerable research attention, studies on its chronic reproductive effects in male mammals remain limited.. Here, we investigated the chronic reproductive toxicity of polystyrene microplastics (PS-MPs, 5 μm) and polystyrene nanoplastics (PS-NPs, 20 nm) both in vitro, using GC2 cells, and in vivo, employing male C57 mice.. Histopathological analysis revealed dilated seminiferous tubules, disorganized spermatocytes, and reduced spermatocyte counts in treated groups.

    Article Title: A Multifunctional Nanoplatform for Treating Hepatic Ischemia–Reperfusion Injury by Modulating the Nrf2 and NF-κB Pathways
    Article Snippet: .. DAPI dihydrochloride, Total Antioxidant Capacity (T-AOC) Assay Kit, Cell Counting Kit-8 (CCK-8), and Colorimetric TUNEL Apoptosis Assay Kit were obtained from Beyotime Biotechnology Company (Shanghai, China). ..

    Apoptosis Assay:

    Article Title: A Multifunctional Nanoplatform for Treating Hepatic Ischemia-Reperfusion Injury by Modulating the Nrf2 and NF-κB Pathways.
    Article Snippet: .. DAPI dihydrochloride, Total Antioxidant Capacity (TAOC) Assay Kit, Cell Counting Kit-8 (CCK-8), and Colorimetric TUNEL Apoptosis Assay Kit were obtained from Beyotime Biotechnology Company (Shanghai, China). ..

    Article Title: Chronic exposure to polystyrene micro/nanoplastics triggers testicular dysfunction through PI3K/AKT/mTOR signaling-mediated spermatocyte senescence in mice.
    Article Snippet: While the potential microplastic toxicity has attracted considerable research attention, studies on its chronic reproductive effects in male mammals remain limited.. Here, we investigated the chronic reproductive toxicity of polystyrene microplastics (PS-MPs, 5 μm) and polystyrene nanoplastics (PS-NPs, 20 nm) both in vitro, using GC2 cells, and in vivo, employing male C57 mice.. Histopathological analysis revealed dilated seminiferous tubules, disorganized spermatocytes, and reduced spermatocyte counts in treated groups.

    Article Title: A Multifunctional Nanoplatform for Treating Hepatic Ischemia–Reperfusion Injury by Modulating the Nrf2 and NF-κB Pathways
    Article Snippet: .. DAPI dihydrochloride, Total Antioxidant Capacity (T-AOC) Assay Kit, Cell Counting Kit-8 (CCK-8), and Colorimetric TUNEL Apoptosis Assay Kit were obtained from Beyotime Biotechnology Company (Shanghai, China). ..

    Staining:

    Article Title: Chronic exposure to polystyrene micro/nanoplastics triggers testicular dysfunction through PI3K/AKT/mTOR signaling-mediated spermatocyte senescence in mice.
    Article Snippet: While the potential microplastic toxicity has attracted considerable research attention, studies on its chronic reproductive effects in male mammals remain limited.. Here, we investigated the chronic reproductive toxicity of polystyrene microplastics (PS-MPs, 5 μm) and polystyrene nanoplastics (PS-NPs, 20 nm) both in vitro, using GC2 cells, and in vivo, employing male C57 mice.. Histopathological analysis revealed dilated seminiferous tubules, disorganized spermatocytes, and reduced spermatocyte counts in treated groups.

    Article Title: A Porcine Monoclonal Antibody Targeting a Conserved GP4 Epitope Protects against In Vivo Infection via the Induction of Broad‐Spectrum PRRSV Neutralization
    Article Snippet: After three additional washes, the cells were incubated with hybridoma supernatants (100 μL per well) at 37 °C for 1 h. The cells were subsequently washed again and labeled with Alexa Fluor 488‐conjugated goat anti‐mouse IgG (H&L chain specific, 1:500 dilution; Thermo Fisher Scientific) for 1 h at 37 °C. .. Simultaneously, the nuclei were stained with DAPI dihydrochloride (Beyotime, C1006) at 25 °C for 15 min. .. Finally, the cells were visualized via an inverted fluorescence microscope (Leica AF6000, Germany) with appropriate filter sets.

    Article Title: A Porcine Monoclonal Antibody Targeting a Conserved GP4 Epitope Protects against In Vivo Infection via the Induction of Broad-Spectrum PRRSV Neutralization.
    Article Snippet: After three additional washes, the cells were incubated with hybridoma supernatants (100 μL per well) at 37 °C for 1 h. The cells were subsequently washed again and labeled with Alexa Fluor 488-conjugated goat anti-mouse IgG (H&L chain specific, 1:500 dilution; Thermo Fisher Scientific) for 1 h at 37 °C. .. Simultaneously, the nuclei were stained with DAPI dihydrochloride (Beyotime, C1006) at 25 °C for 15 min. .. Finally, the cells were visualized via an inverted fluorescence microscope (Leica AF6000, Germany) with appropriate filter sets.

    Article Title: Biomimetic nanodelivery system for precise elimination of tumor-infiltrating Escherichia coli and enhanced treatment of colorectal cancer
    Article Snippet: The development of colorectal cancer is closely related to the abnormal colonization of specific microorganisms in the tumor microenvironment.. To address the challenges of exacerbated carcinogenesis and therapeutic resistance caused by Escherichia coli colonization in colorectal tumors, an innovative pHand targeting-dual-responsive biomimetic nanodelivery system (CIP@EMV@CaCO3) based on bacterial membrane biomimetic strategies was developed.. This system employs E. coli-derived membrane vesicles (EMVs) as carriers to achieve dual functions of homologous targeting for antibacterial purposes and immune activation, overcoming the low delivery efficiency bottleneck of traditional antibiotics.

    cDNA Synthesis:

    Article Title: Chronic exposure to polystyrene micro/nanoplastics triggers testicular dysfunction through PI3K/AKT/mTOR signaling-mediated spermatocyte senescence in mice.
    Article Snippet: While the potential microplastic toxicity has attracted considerable research attention, studies on its chronic reproductive effects in male mammals remain limited.. Here, we investigated the chronic reproductive toxicity of polystyrene microplastics (PS-MPs, 5 μm) and polystyrene nanoplastics (PS-NPs, 20 nm) both in vitro, using GC2 cells, and in vivo, employing male C57 mice.. Histopathological analysis revealed dilated seminiferous tubules, disorganized spermatocytes, and reduced spermatocyte counts in treated groups.

    Bioprocessing:

    Article Title: Chronic exposure to polystyrene micro/nanoplastics triggers testicular dysfunction through PI3K/AKT/mTOR signaling-mediated spermatocyte senescence in mice.
    Article Snippet: While the potential microplastic toxicity has attracted considerable research attention, studies on its chronic reproductive effects in male mammals remain limited.. Here, we investigated the chronic reproductive toxicity of polystyrene microplastics (PS-MPs, 5 μm) and polystyrene nanoplastics (PS-NPs, 20 nm) both in vitro, using GC2 cells, and in vivo, employing male C57 mice.. Histopathological analysis revealed dilated seminiferous tubules, disorganized spermatocytes, and reduced spermatocyte counts in treated groups.

    other:

    Article Title: Pannexin1 Inhibition Suppresses Porphyromonas gingivalis-Induced Ferroptosis in Peri-Implantitis by Regulating the NRF2/SLC7A11/GPX4 Pathway.
    Article Snippet: The cells were incubated overnight at 4°C with the subsequent primary antibodies: Panx1 (1:200, A5167; ABclonal, China), GPX4 (1:200, bs- 61552R; Bioss, China), and FTH1 (1:200, ab65080; Abcam, USA).

    Marker:

    Article Title: Biomimetic nanodelivery system for precise elimination of tumor-infiltrating Escherichia coli and enhanced treatment of colorectal cancer
    Article Snippet: The development of colorectal cancer is closely related to the abnormal colonization of specific microorganisms in the tumor microenvironment.. To address the challenges of exacerbated carcinogenesis and therapeutic resistance caused by Escherichia coli colonization in colorectal tumors, an innovative pHand targeting-dual-responsive biomimetic nanodelivery system (CIP@EMV@CaCO3) based on bacterial membrane biomimetic strategies was developed.. This system employs E. coli-derived membrane vesicles (EMVs) as carriers to achieve dual functions of homologous targeting for antibacterial purposes and immune activation, overcoming the low delivery efficiency bottleneck of traditional antibiotics.

    Clinical Proteomics:

    Article Title: Biomimetic nanodelivery system for precise elimination of tumor-infiltrating Escherichia coli and enhanced treatment of colorectal cancer
    Article Snippet: The development of colorectal cancer is closely related to the abnormal colonization of specific microorganisms in the tumor microenvironment.. To address the challenges of exacerbated carcinogenesis and therapeutic resistance caused by Escherichia coli colonization in colorectal tumors, an innovative pHand targeting-dual-responsive biomimetic nanodelivery system (CIP@EMV@CaCO3) based on bacterial membrane biomimetic strategies was developed.. This system employs E. coli-derived membrane vesicles (EMVs) as carriers to achieve dual functions of homologous targeting for antibacterial purposes and immune activation, overcoming the low delivery efficiency bottleneck of traditional antibiotics.

    Membrane:

    Article Title: Biomimetic nanodelivery system for precise elimination of tumor-infiltrating Escherichia coli and enhanced treatment of colorectal cancer
    Article Snippet: The development of colorectal cancer is closely related to the abnormal colonization of specific microorganisms in the tumor microenvironment.. To address the challenges of exacerbated carcinogenesis and therapeutic resistance caused by Escherichia coli colonization in colorectal tumors, an innovative pHand targeting-dual-responsive biomimetic nanodelivery system (CIP@EMV@CaCO3) based on bacterial membrane biomimetic strategies was developed.. This system employs E. coli-derived membrane vesicles (EMVs) as carriers to achieve dual functions of homologous targeting for antibacterial purposes and immune activation, overcoming the low delivery efficiency bottleneck of traditional antibiotics.



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing